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Why Choukroun's A-PRF?

Many recent studies have shown the interest and potential of white cells in the inflammatory cascade, as a corollary, a prominent action in the early days of stimulation of osseo-progenitor cells. (Omar & Thomsen, Biomaterials, 2012, Kawazoe T, Cell. Transplant 2012)

It was therefore natural to try to capture the whole amount of monocytes in the PRF, to make it more active in stimulating bone grafts, but also to turn to a more rapid transformation of monocytes into macrophages to increase the effect bone stimulation.

This is done with the A-PRF™ or Advanced-PRF™.

The Whole Protocol Changes!

The tubes, the centrifuge rpm and time of spin.

The first results of our research, conducted at the Laboratory of Clarion Research Group, Pennsylvania University (USA) and Repair-Lab, Institute of Pathology, Johannes Gutenberg University, Mainz (Germany) show a release of BMP-2 (Bone Morphogenetic Protein), and BMP-7. These studies also show a greater release of VEGF. Obviously, this release is from the monocytes.

A specific tube that was developed following our scientific research and certified as Medical Device is the tube A-PRF™ +.  

The sterile A-PRF™ + tube is the only authorized at this moment. Conventional tubes being used such as tubes for laboratory tests are now non-regulatory.

The first clinical data and results are very exciting!
Many scientific studies are underway: The results will be published shortly. But the presence of BMP in sufficient quantity, already provides the A-PRF ™ a future by providing practitioners with a very powerful product for use in bone reconstruction.


  • Sinus lift
  • Implants
  • Bone Grafting
  • Healing
  • Periodontology
  • Extractions

Development of i-PRF

Numerous scientific publications describing the action of the white cells on vascularization and wound healing have been published in recent years.

The unanimous conclusion has been that platelet concentrates enriched leukocytes are more effective on tissue and bone healing.

The publication of our research on the A-PRF (PRF enriched leukocytes) and clinical outcomes confirm absolutely this scientific position.
However, the use of platelet concentrates in a “liquid” and not coagulated form remains an important indication in various medical and dental applications.
That is why we have focused our research to a liquid “blood concentrate” enriched in white blood cells, but also platelet-enriched to increase the healing properties while retaining the principle of centrifugation “without anticoagulants” or “no additives “.
The research was led by Dr Joseph Choukroun, the inventor of the PRF technique with  collaboration of two laboratories among the most renowned in the world (FORM in Frankfurt, Germany and Research Lab Clarion in Clarion, USA).

The result is the new protocol i-PRF!

Research of i-PRF (“i” as injectable) has been directed towards obtaining a blood concentrate with very high leukocyte content but which coagulates few minutes after the end of spin.
This “super PRF” is produced with new tubes i-PRF specific for this preparation.
The use of i-PRF is in its infancy, but already, the results in both oral surgery in regenerative medicine are very promising injection into soft tissue to increase vascularity and perhaps improve the biotype, injection into the bone graft particles (biomaterials) to coagulate and get a “solid” free of all movements granules.
The interest of the graft clot also lies in how to carve a bone graft and give it a compact form.
The protocol i-PRF is a real scientific and clinical innovation and will allow many practitioners to improve their results in bone grafts.

PRF DUO Centrifuge

The PRF DUO System is now available HERE